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Stages in contrast enhancement

Stages in contrast enhancement using the VE-DIC method to image unstained microtubules assembled from pure tubulin subunits.

View by eye of microtubules bound to the surface of a coverslip.
Live video image after analog contrast enhancement.
Background image taken just below microtubule focus.
Live video image after subtraction of background image (stored in a frame
buffer).
Live video image after further digital contrast enhancement.

Salmon, E. D. 1995. Video-enhanced differential interference contrast (VE-DIC) light microscopy and the discovery of kinesin. Trends in Cell Biol. 5:154-158.

Other Images: Click on an link below for an image and description.
Newt Lung Epithelial Cell	Pharynx (mouth region) of C. Elegans
Mitotic spindle from sea urchin egg	Mitosis in tissue-cultured newt lung cell
Chinese Hamster Chromosomes	Birefringent structure in living growth cone
VE-DIC of mitotic newt lung epithelial cells: at low and high magnification	Stages in contrast enhancement

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